凌连生的个人简介
凌连生，男，1972年12月生，湖北郧西人，博士，中山大学化学学院教授、分析化学专业博士生导师。
人物经历
教育经历1991.9―995.7：湖北师范学院化学系, 获学士学位；
1995.9―2000.7：武汉大学化学系（硕博连读）获博士学位。导师: 曾云鹗教授；
2000.7―2002.12：中国科学院化学研究所博士后。 导师: 白春礼院士；
2003.1―2004.7：德国马普高分子研究所(MPIP-Mainz，Germany) 博士后；导师：Prof. Dr. Hans-Juergen Butt。
工作经历2005.3―2011.12：中山大学化学与化学工程学院副教授；
2006年入选广东省高等学校“千百十工程”校级培养对象；
2006年11月被遴选为分析化学专业博士生导师；
2012.1至今：中山大学化学与化学工程学院教授, 博士生导师。
讲授课程
《传感器与检测技术》、《光学分析化学》、《仪器分析实验》和《分析化学实验》。
科研方向
分子光谱分析，纳米分析化学，生物分析化学。
学术成果
科研项目作为负责人主持的科研项目：
1.国家自然科学基金, 三螺旋DNA及其与蛋白质(酶)相互作用的纳米光学传感器的研究 (No:20975116).
2. 国家自然科学基金, 基于三螺旋DNA的纳米生物传感器的研究 (No: 20645002)
3. 教育部留学回国人员启动基金, 基于金纳米粒子的DNA纳米生物传感器的研究(2008.1-2009.12)
4. 中山大学“百人计划”启动经费
5.中国博士后科学基金的单个DNA分子凝聚的动力学研究(一等)
6. 中国科学院王宽诚博士后工作奖励基金
7.北京分子科学国家实验室开放课题(2012.10-2014.9)
论著一览2013年
1. Xiao, Z. Y., Guo, X. T., Ling, L. S.* Sequence-specific recognition of double-stranded DNA with molecular beacon with the aid of Ag+ under neutral pH environment. Chem. Commun., 2013, 49 (34), 3573 - 3575.
2. Miao, X. M., Ling, L.S.,* Shuai, X.T.* Sensitive detection of glucose in human serum with Oligonucleotide modified gold nanoparticles by using dynamic light scattering technique. Biosensor and Bioelectronics, 2013, 41, 880-883.
3. Xiong, C., Ling, L.S.* Localized Surface Plasmon Resonance Light Scattering Sensor for Mercury(II) ion with Label-free Gold Nanoparticles. Journal of Nanoscience and Nanotechnology. 2013, 13, 1406-1410.
2012年
4. Li, R.M., Xiong, C., Xiao, Z. Y., Ling, L.S.,* Colorimetric Detection of Cholesterol with G-Quadruplex-Based DNAzymes and ABTS2-. Analytica Chimica Acta. 2012, 724, 80-85.
5. Xiong, C., Xiao, Z. Y., Zhang, M. J., Ling, L.S.* Sensitive detection of H2O2 and H2O2 - related reactant with Ru(bipy)2(7,8-dimethyl-dipyridophenazine)2+ and Oligodeoxyribonucleotide. Analyst, 2012, 137, 4428 u2013 4434.
6. Miao, X. M., Ling, L.S.,* Cheng，D., Shuai, X.T.* Highly sensitive sensor for Cu2+ with unmodified gold nanoparticles and DNAzyme by using dynamic light scattering technique. Analyst, 2012, 137 (13), 3064 u2013 3069.
7. Xiong, C., Ling, L.S.* Label-free, sensitive detection of Hg(II) with gold nanoparticles by using dynamic light scattering technique. Talanta, 2012, 317-321.
8. Miao, X. M., Ling, L.S.,* Shuai, X.T.* Detection of Pb2+ at attomole level by using dynamic light scattering and unmodified gold nanoparticles. Analytical Biochemistry, 2012，421, 582-586.
2011年
9. Miao, X. M., Ling, L.S.,* Shuai, X.T.* Ultrasensitive detection of lead(II) with DNAzyme and gold nanoparticles probes by using a dynamic light scattering technique. Chem. Commun., 2011, 47 (14), 4192 u2013 4194.
10. Miao, X. M., Xiong, C., Wang, W.W., Ling, L.S.,* Shuai, X.T.* Dynamic Light Scattering-Based Sequence Specific Recognition of Double Stranded DNA with Oligonucleotide Functionalized Gold Nanoparticles. Chemistry - A European Journal, 2011, 17, 11230-11236.
11. Wu, C.K., Zhou, Y. M., Miao, X. M., Ling, L. S.* A novel fluorescent biosensor for sequence specific recognition of double-stranded DNA with the platform of graphene oxide. Analyst, 2011, 136 (10), 2106 u2013 2110.
12. Xiong,C., Wu, C. K., Zhang, H., Ling, L.S.* Gold nanoparticles-based Colorimetric investigation of triplex formation under weak alkalic pH environment with the aid of Ag+. SPECTROCHIM ACTA A., 2011, 79, 956u2013961.
13. 吴呈珂，董秋鹏，张红，熊岑，吴秀丽，凌连生*, [Cu(DPPZ)(L-Ser)]+与DNA的相互作用及其分析应用研究. 光谱学与光谱分析，2011，31(4),1057-1060.
2010年
14. Wu C.K., Xiong, C., Wang, L., Lan, C., Ling, L.S.* Sensitive and Selective Localized Surface Plasmon Resonance Light-Scattering Sensor for Ag+ with Unmodified Gold Nanoparticles. Analyst, 2010, 135, 2682u20132687.
2009年
15. Xin, A., Dong, Q. P., Xiong, C., Ling, L. S.* Colorimetric recognition of DNA intercalators with unmodified gold nanoparticles. Chem. Commun., 2009, 1658-1660.
16. Yan, H. L., Xiong, C., Yuan, H., Zeng, Z. X., Ling, L.S.* Spacer control the dynamic of triplex formation between oligonucleotide modified gold nanoparticles. Journal of Physical Chemistry C. 2009, 113, 17326u201317331.
2008年以前
17. Ling,L.S., Butt,H. J., Berger, R.* The rupture force between the third strand and double strand within a triplex DNA. J. Am. Chem. Soc. (Article) 2004, 126, 13992-13997.
18. Ling,L.S., Butt,H. J., Berger, R.* Rupture Force Changes between the Third Strand and the Double Strand within a Triplex DNA in the Presence of Intercalative Molecules. Appl. Phys. Lett.. 2006, 89(11), 113902.
19. Ling,L.S., Jiang, Y. X., Wang, C., Fang, X. H.*, Wan, L. J., He, Z. K., Bai, C. L.*, Chen, D. M.* Delaying Photobleaching and Recovering Luminescence of a Molecular Light Switch in DNA Analysis. Analytical Biochemistry, 2004, 329, 334-336.
20. 凌连生, 汪俊，方晓红，陈东敏，白春礼*, 三螺旋脱氧核糖核酸的研究进展 (评述). 分析化学, 2004 32, 1252-1255.
21. Yang, G. Z., Ling,L.S., Wan,L.J.*, Fang,X.H.,* Bai, C.L.* Electrochemical Scanning Tunneling Microscope Imaging of Self-Assembed Monolayer of Double-Stranded DNA on Au(111). Journal of Nanoscience and Nanotechnology, 2004, 4(6), 561-564.
22. Li, B. S., Kang,S. Z., Cheuk,K.K.L., Wan, L.J., Ling,L.S. Bai,C.L.* Tang,B. Z.* Self-assembling of Helical Poly(Phenylacetylene) Carrying L-Valine Pendants in Solution, on Mica Substrate, and on Water Surface，Langmuir, 2004, 20, 7598-7603.
23. Ling,L.S., He, Z. K.*, Chen, F., Zeng, Y. E. Single-mismatch detection using nucleic acid molecular "’light switch"’. Talanta, 2003, 59 (2), 269-275.
24. Ling, L.S., Fang, X.H., Wang, C., Wan, L.J., Bai, C.L.* Chen, D.M., Direct observation of the DNA multimolecular condensation with fluorescence microscopy, Chemistry Letters, 2003, 32 (1), 80-81.
25. Ling,L.S., He, Z. K.*, Chen, F., Zhang, H. S., Zeng, Y. E. Study on triplex DNA by use of molecular "Light Switch" complex of Ru(phen)2(dppx)2+. Chinese Chemical Letters. 2003, 14
(3), 300-303.
26. Jiang, Y.X., Zhu, C.F., Ling, L.S., Wan, L. J., Fang, X. H.*, Bai, C. L.* Specific Aptamer - Protein Interaction Studied by Atomic Force Microscopy, Analytical Chemistry, 2003, 75, 2112-2116.
27. Li, B. S., Cheuk, K. K. L., Ling,L.S., Chen, J. W., Xiao, X. D., Bai, C. L.*, Tang, B. Z.* Synthesis and Hirarchical structures of amphilic polyphonylacetylenes carrying L-valine pendents. Macromolecules, 2003, 36, 77-85.
28. Ling,L.S.*, He, Z. K., Song, G. W., Zeng, Y., Wang, C., Bai, C. L., Chen, X. D., Shen, P. High sensitive determination of DNA by use of molecular"Light Switch" complex of Ru(phen)2(dppx)2+ Analytica Chimica Acta.2001, 436(2), 207-214.
29. Yang, J. L., Lin, H. Z., He, Q. G., Ling,L.S., Zhu, C. F., Bai, F. L.* Composition of hyperbranched conjugated polymers with nanosized cadium sulfide particles, Langmuir, 2001, 17(19), 5978-5983.
30. 凌连生，何治柯*，曾云鹗, 核酸荧光探针的研究进展 (评述)，分析化学， 2001, 29 (6), 721-724.
31. Ling,L.S., He, Z. K.*, Song, G. W., Zeng,Y. E. A novel method for determination of DNA by use of molecular "Light Switch" complex of Ru(bipy)2(dppx)2+ . Analytica Chimica Acta. 2000, 403 (1-2), 209-217.
32. Ling,L.S., He, Z. K.*, Song, G. W., Zeng,Y. E. Determination of DNA by use of the molecular"Light Switch"complex of Ru(bipy)2(dppz)2+. Mikrochimica Aata 2000, 134(1-2), 57-62.
33. 凌连生，何治柯*，吴凤武，罗庆尧，曾云鹗，核酸分子 "光开关" 的研究进展 (评述). 高等学校化学学报， 2000, 21 (4), 527-531.
34. Li, X.Y., Ling,L.S., He, Z. K.*, Zeng,Y. E. Development of a Chemiluminescence method for the simultaneous determination of pyruvic and tartaric acids in human serum based upon their reaction with cerium(IV) in the presence of rutheniumtrispyridine. Microchemical Journal. 2000, 64, 9-13.
35. Ling,L.S., He, Z. K.*, Song, G. W., Zeng,Y. E. Spectral studies on the interaction of Ru(phen)2(dppx)2+(phen=1,10-phenanthroline, dppx=7,8u2013dimethyl -dipyridophenazine) and DNA. SPECTROSCOPY LETT，1999, 32 (6), 931-939.
36. Ling,L.S., Song, G. W., He, Z. K.*, Zeng,Y. E. A novel method to determine DNA by use of molecular "Light Switch" complex of Ru(phen)2(dppz)2+. Microchemical Journal. 1999, 63 (3), 356-364.
37. Ling,L.S., He, Z. K.*, Zeng, Y. E. Spectral studies on the interaction of DNA and Ru(bipy)2(dppx)2+.SPECTROCHIM ACTA A. 1999, 55 (6), 1297-1302.
38．He, Z. K.*, Wu, F. W., Meng, H., Ling,L.S., Zeng,Y. E. Chemiluminescence determination of thiorea using tris(2,2u2019-bipyridyl) ruthenium (II)-KMnO4 system. Analytical Sciences. 1999, 15 (4), 381-383.
39. 何治柯*, 凌连生, 蔡汝秀,曾云鹗， Ru(2,2u2019-bipyridine)32+-α-羟基酸-Ce(IV) 化学发光反应机理研究，化学学报，1998, 56 (5), 472-477.
40. 凌连生, 何治柯*, 蔡汝秀, Ru(bipy)32+-Ce(IV)-丙酮酸体系测定血清中的丙酮酸, 高等学校化学学报，1997,18(12),1963-1965.
