金双侠

时间:2023-12-17 04:00:29编辑:资料君

金双侠的个人简介

华中农业大学植物科学技术学院作物遗传育种专业博士,教授,博士生导师。主要从事棉花生物技术研发(基因编辑等);棉花与害虫互作分子机制及抗虫相关基因的克隆和高效表达载体(叶绿体转化表达体系)的开发研究等; 高等植物基因组进化。

基本概况

姓名:金双侠

硕博导:博导

职称:教授

开设课程:植物生物技术,作物生产实习,育种学实习

研究方向:目前主要从事棉花生物技术研发(基因编辑等);棉花与害虫互作分子机制及抗虫相关基因的克隆和高效表达载体(叶绿体转化表达体系)的开发研究等; 高等植物基因组进化。

学习和工作经历

1997-2001年,华中农业大学 学士

2001-2006年,华中农业大学 硕士、博士学位

2007.10?-2012.4?, 美国宾夕法尼亚大学Henry Daniell?教授课题组进行博士后研究

2012.5- 至今, 华中农业大学作物遗传改良国家重点实验室PI, 博导、教授

个人简介

华中农业大学植物科学技术学院教授、博导,作物遗传改良国家重点实验室PI, 塔里木大学“昆仑学者”特聘教授。

近年来以第一作者或通讯作者在国际知名学术期刊 GenomeBiology, Advanced Science, Nature Communications, Trends in Plant Science,New Phytologist,Plant Physiology,Plant Biotechnology Journal?等杂志上发表30多篇植物抗虫基因工程的学术文章,其中IF大于6的文章18篇,论文被引用次数2300多次,H指数:29。2018年入选?Plant Biotechnology Journal?杂志(IF=8.15)副主编以及Journal of Cotton Research(棉花学报英文版)副主编(高级编辑);2017年当选棉花基础研究领域权?[2]威学术机构-国际棉花基因组协会(ICGI)共同主席。先后主持国家自然科学基金面上项目4项,转基因专项子课题3项,十三五国家重点研发专项(子课题)1项,湖北省自然科学基金重点项目(杰青)1项 。

科研项目

1.国家自然科学基金 面上项目主持2020-2024十三五国家重点研发专项 子课题 主持 2016-2020

3. 转基因专项子课题(3项) 主持 2016-2020

4. 湖北省自然科学基金重点项目(杰青项目) 主持 , 2015.1-2016.12

5. 国家自然科学基金 面上项目 烟粉虱与棉花互作的分子机理研究 主持,2014-2017

6. 国家自然科学基金 面上项目 植物凝集素基因在棉花中的功能分析, 主持, 2012-2015

7. 新疆建设兵团博士资金项目 转基因抗病虫、抗逆聚合杂交棉新组合选育技术研究,主持,2007-2009

8. 国家自然科学基金 面上项目 棉花外源基因叶绿体转化体系的建立及其应用(30771368),主持,2008-2010

9.教育部博士点新教师基金 以gfp基因为报告基因监测转基因棉花花粉漂移(20070504087),主持,2008-2010

10. 国家自然科学基金重大国际合作项目 Development of strong pest-resistance cotton viatransforming plastid and

its application in breeding, 主要参加者,2009-2011

11. 国家自然科学基金 利用基因诱捕构建棉花功能基因组研究突变群体,主要参加,2005-2007

获奖情况及发明专利

获奖情况

【棉花种质创新及强优势杂交新品种选育与应用】 国家科技进步二等奖 2013年

【棉花体细胞离体遗传操作技术与控制理论】 教育部自然科学二等奖

湖北省优秀博士学位论文(第10批)获奖者,2008年

湖北省优秀本科论文指导奖,2008年。指导的2007界本科生张碧瑶,其论文:新疆棉花高体细胞胚胎发生

能力基因型的筛选,获得湖北省优秀本科学位论文

“棉花分子育种技术体系建立与应用”获2008年湖北省技术发明二等奖

获第十、十一届湖北省自然科学优秀论文一等奖2次

申报六项发明专利

1. 超声波辅助农杆菌转化棉花胚芽的方法,专利申请号:200710063650.0

2. 一种分离棉花叶绿体DNA的方法,专利申请号:200710052665.7

3.利用苦豆子分离基因SaVP1培育耐高温拟南芥的方法. 专利号ZL 201310006089.8

4.利用花花柴KcNHX1 基因培育耐高温拟南芥的方法 专利申请号:201310160491.1

5. 利用棉花GhPEPC基因创造棉花高油材料的方法 专利申请号:201510836230.6

6. 一种提高棉花再生及转化效率的方法及应用 专利申请号 201510836230.6

7. 一种棉花基因的编辑方法 专利申请号 2016111884024

8. 利用植物介导的RNAi技术创造棉花抗中黑盲蝽新种质的方法 专利申请号:2017102984371

9. 一种DsRED2报告基因在棉花遗传改良中的应用 专利申请号 201611113581.5

10. 陆地棉基因组的精准高效编辑方法 专利申请号2018115777177

11. 陆地棉基因组的精确高效编辑方法 专利申请号 201910290886.0

12. 利用植物介导的RNAi创造棉花抗绿盲蝽种质的方法? 专利申请号 2019100115879

发表的代表性论文

1. Bo Li, Sijia Liang, Muna Alariqi, Fuqiu Wang, Guanying Wang, QiongqiongWang1, Zhongping Xu, Lu Yu, Muhammad Naeem Zafar, Lin Sun, Huan Si, DaojunYuan, Weifeng Guo, Yanqin Wang, Keith Lindsey, Xianlong Zhang*,?Shuangxia Jin*。Theapplication of temperature sensitivity CRISPR/LbCpf1 (LbCas12a) mediated genomeediting in allotetraploid cotton (G. hirsutum) and creation of nontransgenic,gossypol-free cotton.?PlantBiotechnology Journal,?2020. (*通讯作者)(IF: 8.15)

2. Junzhong Shang, Jingpu Tian, Huihui Cheng, Qiaomu Yan, Lai Li, AbbasJamal, Zhongping Xu, Lin Xiang, Christopher A Saski,?Shuangxia Jin*,?Kaige Zhao*, Xiuqun Liu*, Longqing Chen*.The chromosome-level wintersweet (Chimonanthus praecox) genome provides insights into floral scent biosynthesis and flowering in winter.?Genome Biology,?2020?(*通讯作者)(IF: 10.8)

3. Qiuyue Ma, Tianlin Sun, Shushun Li, Jing Wen, LuZhu, Tongming Yin, Kunyuan Yan, Xiao Xu, Shuxian Li, Jianfeng Mao, Ya-nan Wang,?Shuangxia Jin, Xing Zhao*, Qianzhong Li* . The Acer truncatum genome provides insights into the nervonic acid biosynthesis.?Plant Journal, 2020.?(IF: 6.14)

4. Qiongqiong Wang, Muna Alariqi, Fuqiu Wang, Bo Li, Xiao Ding, HangpingRui, Yajun Li, Jianying Li, Zhongping Xu, Lei Qin, Lin Sun, Jianying Li, JiaweiZou, Keith Lindsey, Xianlong Zhang,?ShuangxiaJin*.?The application of a heat-inducible CRISPR/Cas12b (C2c1) genome editing system in tetraploid cotton (G.hirsutum) plants.?PlantBiotechnology Journal,2020 (*通讯作者)(IF: 8.15)

5.?Hakim Manghwar, Xianlong Zhang*,?ShuangxiaJin*. Strategies to overcome off-target effects by CRISPR/Cas system (2020).?Advanced Science. 2020, 7(6):1902312 (*通讯作者)(IF: 15.8)

6.?Hakim Manghwar, Xianlong Zhang*,?ShuangxiaJin*. CRISPR/Cas system: its recent advances and future prospect in genome editing of plant species (2019),?Trends in Plant Science. 2019, 24(12):1102-1125(*通讯作者)(IF: 14.4)

7.Lisong Hu, Zhongping Xu, et al., Chaoyun Hao* &?Shuangxia Jin*.?The chromosome-scale reference genome of black pepper (Piper nigrum) provides further insight into piperine biosynthesis.?Nature Communications,2019, 10:4702(*通讯作者)(IF: 12.1)

8.Lei Qin, Jianying Li, Qiongqiong Wang, Zhongping Xu, Lin Sun, Muna Alariqi, HakimManghwar, Guanyin Wang, Bo Li, Xiao Ding, Hangping Rui, HuimeiHuang, Tianliang Lu , Keith Lindsey,HenryDaniell, Xianlong Zhang,?Shuangxia Jin*. High Efficient and Precise Base Editing of C?G to T?A in the Allotetraploid Cotton (Gossypium hirsutum) Genome Using a Modified CRISPR/Cas9 System.?Plant Biotechnology Journal,?2020, 18(1):45-56 (*通讯作者)(IF: 8.15)

9.Bo Li, Hangping Rui, Yajun Li, Qiongqiong Wang, Muna Alariqi, Lei Qin, Lin Sun, Xiao Ding, Fuqiu Wang, Jiawei Zou,YanqingWang, Daojun Yuan, Xianlong Zhang?ShuangxiaJin?*. Robust CRISPR/Cpf1(Cas12a) mediated genome editing in allotetraploid cotton (G.hirsutum).?Plant Biotechnology Journal,?2019, 17(10):1862-1864(*通讯作者)(IF: 8.15)

10. Maojun Wang, Lili Tu, DaojunYuan, De Zhu, Chao Shen, Jianying Li, Fuyan Liu, Liuling Pei, Pengcheng Wang,Guannan Zhao, Zhengxiu Ye, Hui Huang, Feilin Yan, Yizan Ma, Lin Zhang, Min Liu,Jiaqi You, Yicheng Yang, Zhenping Liu, Fan Huang, Baoqi Li, Ping Qiu, QinghuaZhang, Longfu Zhu,?Shuangxia Jin,?Xiyan Yang, Ling Min, Guoliang Li,Ling-Ling Chen, Hongkun Zheng, Keith Lindsey, Zhongxu Lin, Joshua A. Udall& Xianlong Zhang. Reference genome sequences of two cultivated allotetraploid cottons,?Gossypium hirsutum?and?Gossypium barbadense.?Nature Genetics,?2019, 51(2): 224-229(IF:27.6)

11.Li Jianying; ManghwarHakim; Sun, Lin; Wang, Pengcheng; Wang, Guanying; Sheng, Hanyan; Zhang, Jie; Liu, Hao; Qin, Lei; Rui, Hangping; Li, Bo, Lindsey, Keith, Daniell, Henry,Jin, Shuangxia*; Zhang, Xianlong.Whole genome sequencing reveals rare off-target mutations and considerable inherent genetic or/and somaclonal variations in CRISPR-Cas9-edited cotton plants.?Plant Biotechnology Journal,2019, 17, 858-868 (*通讯作者)(IF: 8.15)

12. Jianying Li, Maojun Wang, YajunLi, Qinghua Zhang, Keith Lindsey, Henry Daniell,?Shuangxia Jin*,Xianlong Zhang. Multi-omics analyses reveal epigenomics basis for cotton somatic embryogenesis through successive regeneration acclimation (SRA)process,?Plant Biotechnology Journal,?2019, 17, 435u2013450(*通讯作者)?( IF: 8.15)

13. Hu Q, Min L, Yang X,?Jin S,Zhang L, Li Y, Ma Y, Qi X, Li D, Liu H, Lindsey K, Zhu L, Zhang X. LaccaseGhLac1 modulates broad-spectrum biotic stress tolerance via DAMP-triggered immunity.?Plant Physiology, 2018, 176(2):1808-1823.?(IF: 6.9)

14. Wang, Pengcheng; Zhang, Jun;Sun, Lin; Ma, Yizan; Xu, Jiao; Liang, Sijia; Deng, Jinwu; Tan, Jiafu; Zhang,Qing; Tu, Lili; Daniell, Henry;?Jin, Shuangxia*; Zhang, Xianlong*. High efficient multi-sites genome editing in allotetraploid cotton (Gossypiumhirsutum) using CRISPR/Cas9 system,Plant Biotechnology Journal,?2018,16, 137-150(*通讯作者)(IF: 8.15)

15. Luo, Jing; Liang, Sijia; Li,Jianying; Xu, Zhongping; Li, Lun; Zhu, Bangqin; Li, Zhe; Lei, Chaoliang;Lindsey, Keith; Chen, Lizhen*;?Jin, Shuangxia*; Zhang, Xianlong. A transgenic strategy for controlling plant bugs (Adelphocoris suturalis) through expression of double- stranded RNA (dsRNA) homologous to Fatty acyl-CoA reductase (FAR) in cotton.?New Phytologist,?2017,215,1173-1185(*通讯作者)?( IF: 8.5)

16. Maojun Wang, Lili Tu, Min Lin,Zhongxu Lin, Pengcheng Wang, Qingyong Yang, Zhengxiu Ye, Chao Shen, JianyingLi, Lin Zhang, Xiaolin Zhou, Xinhui Nie, Zhonghua Li, Kai Guo, Yizan Ma, CongHuang,?Shuangxia Jin,?Longfu Zhu, Xiyan Yang, Ling Min, Daojun Yuan,Qinghua Zhang, Keith Lindsey & Xianlong Zhang. Asymmetric subgenome selection and cis-regulatory divergence during cotton domestication,?Nature Genetics,?2017,49(4):579-587 (IF: 27)

17. Jianying Li#, Lizhen Zhu#, Joe Hull, SijiaLiang, Henry Daniell,?Shuangxia Jin*,?Xianlong Zhang. Transcriptome analysisreveals a comprehensive insect resistance response mechanism in cotton to infestation by the phloem feeding insect Bemisia tabaci (whitefly).?PlantBiotechnology Journal,?2016,14,1956-1975 (SCI, IF:6.3)(通讯作者)(IF: 8.15)

18.?Shuangxia Jinand Henry Daniell.The Engineered Chloroplast Genome Just Got Smarter.?Trendsin Plant Science, 2015,20(10):622-640 (IF:14.4)

19.?Jin Shuanxia,Singh, Dolendro,Li Lebin,Zhang, Xianlong and Daniell, Henry. Engineered chloroplast dsRNA silences cytochrome p450 monooxygenase, V-ATPase and chitinsynthases genes in the insect gut and disrupts Helicoverpa armigera larval development and pupation.?Plant Biotechnology Journal, 2015,13(3), 435-446. (IF:8.15)

20.?Shuangxia Jinand Henry Daniell. Expression of γ-tocopherol methyltransferase in chloroplasts results in massive proliferation of the inner envelope membrane and decreases susceptibility tosalt and metal-induced oxidative stress by reducing reactive oxygen species.?Plant Biotechnology Journal,?2014 (12),1274-1285 . (IF:8.15)

21.?Shuangxia Jin,Xianlong Zhang1, Henry Daniell. Pinellia ternata agglutinin expression in chloroplasts confers broad spectrum resistance against aphid, whitefly,lepidopteran insects, bacterial and viral pathogens.?Plant Biotechnology Journal?2012,10(3):313-327 (Cover Story, IF 8.15 ).

22.?Shuangxia Jin,Anderson Kanagaraj, Dheeraj Verma, Theo Lange, Henry Daniell.Release of Hormones from Conjugates: Chloroplast Expression of ?-glucosidase Results in Elevated Phytohormone Levels Associated with Significant Increase in Biomass and Protection from Aphids or Whiteflies Conferred by Sucrose Esters.Plant Physiology?,2011,155,222-235. (IF:6.9).

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